RNAi technology is not only a powerful tool for gene function research, but can also be used to inhibit the expression of pathogenic genes, as a potential therapeutic tool for disease treatment. The success of an RNAi assay is dependent on the following factors:
- Choice of target sequence and siRNA design
- Cell line and cell culture system
- Transfection conditions
- Accumulation and replacement ratio of target gene mRNA
- Target protein half-life
- Accurate detection of mRNA- and protein-levels, and cell phenotype
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GenePharma’s RNAi scientific service team can help clients to achieve maximal value out of their RNAi assays and avoid costly and time-consuming mistakes in experimental setup. Our RNAi service can be applied in the following areas:?functional genomics, gene therapy, drug target screening, cellular signal pathway analysis, protein interaction, and more. Our scientific team will work with you to develop the most extensive and effective RNAi technology solutions to address your specific research questions.
GenePharma’s RNAi services include two separate fields:?
- Chemically-synthetic RNAi service, based on (chemically modified) siRNA;
- Vector based RNAi service.
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Chemically-synthetic RNAi service
Our RNAi services based on chemically-synthetic siRNAs include:
- siRNA design:?customer provides target gene name, sequence or GeneBank ID, etc.;
- siRNA synthesis;
- siRNA transfection into mammalian cells according to the customer’s specifications;?
- Test of inhibitory effect: the mRNA expression is detected by RT-PCR,?the protein expression is detected by Western Blot;
- Provision of experimental reports: siRNA sequence, RT-PCR test reports, Western Blot pictures, and so on.?
Vector-based RNAi Service
Our RNAi services based on vector-expressed shRNAs include:
- shRNA design:?customer provides target gene name, sequence or GeneBank ID, etc.;
- shRNA synthesis;?
- Choice of shRNA vector: for instance, pGPU6/GFP/Neo or pGPH1/GFP/Neo;
- Construction of shRNA vector;
- Vector transfection into mammalian cells according to the customer’s specifications;
- Test of inhibitory effect: the mRNA expression is detected by RT-PCR,?the protein expression is detected by Western Blot;
- Provision of experimental reports: shRNA sequence, sequencing report of the constructed vector, RT-PCR test reports, Western Blot pictures, and so on.?
For all RNAi Services, the fee is based on the requirements of the specific experiments. Reference prices are as follows:
Cat. No. |
Service |
Price (USD) |
Lead Time |
K-01 |
siRNA/shRNA Transfection, RT-PCR, Western Blot |
$ 2,499 |
4 weeks |
K-02 |
siRNA/shRNA Transfection, RT- PCR |
$ 2,249 |
4 weeks |
K-03 |
siRNA/shRNA Transfection, Western Blot? |
$ 2,249 |
4 weeks |
For technical questions, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com. |
