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The?Hairpin-itTM?miRNAs qPCR Quantitation Kit?is a sensitive and specific method using real-time PCR?for the detection and quantification of miRNAs from total RNA samples.?MiRNAs are small, single-stranded, ~19–23 nt RNA molecules encoded in the genomes of plants, animals, and viruses. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Unlike commonly used methods for detection of miRNAs,?the?Hairpin-itTM?miRNAs qPCR Quantitation Kit?is more rapid and sensitive.?
This Kit contains?a miRNA specific RT and PCR primer set, with a Molecular Beacon probe included. The stem-loop-like miRNAs RT primer and the miRNAs specific Beacon probe ensure the RT and PCR reaction would not be interfered by?the?miRNAs?precursors. The reaction template could be total RNA or cell lysates.
To normalize for RNA content among different experimental samples, the?U6?snRNA qPCR Normalization Kit?(Cat #?QPM-050) and?5S rRNA qPCR Normalization Kit?(Cat #?QPM-060) are available separately.
Hairpin-itTM?miRNAs qPCR Quantitation Kit?Reagents?box includes the following items.?Store the components at –20℃.?Reagents?must be stored in the dark.

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Accessory Products
Some of the reagents supplied in the?Hairpin-itTM?miRNAs qPCR Quantitation Kit?as well as other products suitable for use with the kit are available separately from GenePharma. Ordering information is provided below.

For technical questions, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.